Description
Break-apart microwells are coated with monoclonal anti- H. pylori capture antibodies. Patient samples are diluted in H. pylori Stool Diluent and added to the microwells. A monoclonal antibody conjugated to horseradish peroxidase (HRP) is added to the microwells and incubated for 60 minutes at room-temperature. Unbound conjugate is removed by washing. TMB-substrate is added; the substrate is hydrolyzed by the peroxidase and yields a blue solution of the reduced substrate. Upon the addition of the stop solution, the blue color turns yellow and should be read by an ELISA reader at a wavelength of 450/620 nm